What is transcriptome sequencing?

Transcriptome sequencing, or RNA sequencing (RNA-Seq), provides fundamental insights into how genomes are organized and regulated—giving us valuable information about the internal state of cells and how the altered expression of genetic variants contributes to complex diseases research. RNA sequencing relies on next-generation sequencing (NGS) methodology and techniques.

Advantages of RNA-Seq over microarray techniques:

Whole transcriptome sequencing, or RNA-Seq, for both discovery and gene expression analysis. Use the Ion Proton™ System combined with Ambion® RNA purification and Ion Torrent™ library construction kits for identification and quantitation of both known and novel transcripts, including gene fusions and splice variants.

Ion AmpliSeq™ Transcriptome solution for gene-level expression analysis. Ideal for traditional gene expression analysis, the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit targets >20,000 RefSeq transcripts using as little as 10 ng RNA input with a fast and FFPE-compatible workflow.

Increase sensitivity—increase your dynamic range to detect more RNA species as well as fusion transcripts and low-abundance isoforms

Quantify changing expression levels—NGS provides a representation of absolute expression, allowing you to identify and characterize low-abundance transcripts during development and under different conditions

Discover biomarkers—the depth of NGS sequencing coverage enables transcriptome-wide biomarker discovery, linking specific disorders to regions of the human genome in your research.